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Merck

AB5731

Sigma-Aldrich

Anti-Nanog Antibody, NT

Chemicon®, from rabbit

동의어(들):

Homeobox transcription factor Nanog, Nanog homeobox

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

모든 문서 보기
기술 서비스
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도움 문의
기술 서비스
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도움 문의

생물학적 소스

rabbit

Quality Level

100

결합

unconjugated

항체 형태

affinity purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human, rat, mouse

포장

antibody small pack of 25 μg

제조업체/상표

Chemicon®

기술

western blot: suitable

입력

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

동형

IgG

NCBI 수납 번호

NM_024865.2

UniProt 수납 번호

Q9H9S0

배송 상태

ambient

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... NANOG(79923)
mouse ... Nanog(71950)

관련 카테고리

일반 설명

39 kDa (MW 34 kDa)
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

면역원

Epitope: N-terminus
Synthetic peptide from mouse Nanog.

애플리케이션

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

생화학적/생리학적 작용

Antibody recognizes Nanog.

물리적 형태

Affinity purified
Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

제조 메모

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

분석 메모

Control
Whole cell lysate from mouse embryonic stem cells.
Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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문서 라이브러리 방문

A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis.
Kuijk, EW; de Gier, J; Lopes, SM; Chambers, I; van Pelt, AM; Colenbrander, B; Roelen, BA
Testing null
Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells.
Lucia Morgado-Palacin,Susana Llanos,Manuel Serrano
Cell Cycle null
AICAR Induces G1 S Arrest and Nanog Down-Regulation via p53 and Enhances Erythroid Differentiation.
Chae HD, Lee MR, Broxmeyer HE
Stem Cells null
Gaelen S Smith et al.
Biomolecules, 3(3), 351-368 (2014-03-13)
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that activates a family of G protein coupled-receptors (GPCRs) implicated in mammalian development, angiogenesis, immunity and tissue regeneration. S1P functions as a trophic factor for many cell types, including embryonic stem cells (ESCs). Sphingosine
Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
Tsukiyama T, Asano R, Kawaguchi T, Kim N, Yamada M, Minami N, Ohinata Y, Imai H.
Genes Cells null
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