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Merck

AB5320

Sigma-Aldrich

Anti-CSPG4 Antibody

CHEMICON®, rabbit polyclonal

동의어(들):

Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan 3, Melanoma-associated chondroitin sulfate proteoglycan, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated)

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

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기술 서비스
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기술 서비스
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도움 문의

제품 이름

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, Chemicon®, from rabbit

생물학적 소스

rabbit

Quality Level

100

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

rat, mouse, monkey, human

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI 수납 번호

NM_001897.4

UniProt 수납 번호

Q6UVK1

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
rhesus monkey ... Cspg4(713086)

일반 설명

270-300 kDa
Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form.(Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).

면역원

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.

애플리케이션

Detect NG2 Chondroitin Sulfate Proteoglycan using this Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody validated for use in ELISA, IC, IH, IP and WB.
Immunocytochemistry:
A 1:150-1:600 dilution of a previous lot was used in IC.

Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.

Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.

ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions and protocols must be determined by end user
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

생화학적/생리학적 작용

NG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

물리적 형태

Antibody purified by protein A, then placed over a fibronectin affinity column.
Purified rabbit polyclonal in PBS buffer containing 0.02% azide.

제조 메모

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.
Routinely evaluated by Western Blot on rat brain lysates

Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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