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Merck

2504

Millipore

ReBlot Plus Strong Antibody Stripping Solution, 10x

동의어(들):

Western blot stripping solution, blot stripping solution

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
41116010
eCl@ss:
42029053

주요 문서

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기술 서비스
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도움 문의

양식

liquid

포장

pkg of 50 mL

제조업체/상표

Chemicon®
Re-Blot™

기술

western blot: suitable

검출 방법

chemiluminescent

배송 상태

wet ice

관련 카테고리

일반 설명

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

Stripping and re-probing of Western blots offers several advantages:

1) Conservation of samples that are expensive or available only in limited quantities,

2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,

3) Re-analysis of anomalous results and confirmation with the same or a different antibody,

4) Correcting errors in incubation with the wrong antibody,

5) Cost savings in reagents and time by reusing the same blot.

While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.

The MILLIPORE Re-Blot Plus Western Blot Strong Antibody Stripping Solution contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.

Advantages of the Re-Blot Plus Western Blot Strong Antibody Stripping Solution include:

· No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution.

· Antibody stripping is done at room temperature. No heating of blots is required.

· Blots can be stripped of antibodies in approximately 15 minutes at room temperature.

· Blots may be reused in 25 minutes.

애플리케이션

The MILLIPORE Re-Blot Plus Western Blot Strong Antibody Stripping Solution is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site.

The Re-Blot Plus Western Blot Strong Antibody Stripping Solution should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

This product is for research use only; not for diagnostic or in vivo use.

제조 메모

The Strong Antibody Stripping Solution should be stored at 2-8°C upon arrival. Product is stable for 3 to 6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37°C before use.

Note: To prevent reagent degradation secure the cap tightly upon storage. Avoid extended exposure to air.

기타 정보

Strong Antibody Stripping Solution (10x) - (2 containers, 25 mL each).

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Re-Blot is a trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Skull and crossbonesCorrosionEnvironment
GHS06,GHS05,GHS09

신호어

Danger

유해 및 위험 성명서

H290,H302,H311 + H331,H314,H411

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Eva E Nagiec et al.
Antimicrobial agents and chemotherapy, 49(9), 3896-3902 (2005-08-30)
The oxazolidinones are a relatively new structural class of antibacterial agents that act by inhibiting bacterial protein synthesis. The oxazolidinones inhibit mitochondrial protein synthesis, as shown by [35S]methionine incorporation into intact rat heart mitochondria. Treatment of K562 human erythroleukemia cells
Anna Dzionek et al.
Molecules (Basel, Switzerland), 25(4) (2020-02-23)
The naproxen-degrading bacterium Bacillus thuringiensis B1(2015b) was immobilised onto loofah sponge and introduced into lab-scale trickling filters. The trickling filters constructed for this study additionally contained stabilised microflora from a functioning wastewater treatment plant to assess the behavior of introduced
Fiona Robinson et al.
PloS one, 3(3), e1801-e1801 (2008-03-13)
The frequency of synonymous codon usage varies widely between organisms. Suboptimal codon content limits expression of viral, experimental or therapeutic heterologous proteins due to limiting cognate tRNAs. Codon content is therefore often adjusted to match codon bias of the host
Abu Hasan Hassimi et al.
Journal of environmental management, 260, 110046-110046 (2020-02-25)
This study was conducted to examine the production of bioflocculants using agricultural wastewater as a fermentation feedstock under different temperatures and incubation times. The mechanism of flocculation was studied to gain a detailed understanding of the flocculation activity. The highest
Céline Cudejko et al.
Blood, 118(9), 2556-2566 (2011-06-04)
The CDKN2A locus, which contains the tumor suppressor gene p16(INK4a), is associated with an increased risk of age-related inflammatory diseases, such as cardiovascular disease and type 2 diabetes, in which macrophages play a crucial role. Monocytes can polarize toward classically
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프로토콜

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

관련 콘텐츠

Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

SNAP i.d. 2.0 System Brochure

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

Immobilon® Block Noise Cancelling Reagents

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