234154
Human Collagen Type IV
from human placenta, liquid, suitable for cell culture
동의어(들):
Collagen, Type IV, Human Placenta, Native, type IV collagen
제품 이름
Collagen, Type IV, Human Placenta, (100 μg), solution, suitable for cell culture, purified by (Highly purified), Native protein
생물학적 소스
human placenta
Quality Level
설명
Merck USA index - 14, 2480
분석
≥90% (SDS-PAGE)
양식
solution
정제법
(Highly purified)
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
농도
(100 μg)
기술
cell culture | mammalian: suitable
배송 상태
wet ice
저장 온도
−20°C
일반 설명
Collagen Type IV is a primary component of the basement membrane (BM). It is a specialized sheet-like extracellular matrix that surrounds specific cell types such as skeletal muscle cells, smooth muscle cells, heart muscle cells, and adipocytes in multicellular tissues. Collagen type IV is characterized as a nonfibril-forming collagen and is composed of six genetically distinct α-chain polypeptides. These chains have the potential to form 56 different combinations, resulting in various homotrimers. Through self-assembly, collagen type IV constructs organized networks that play a crucial role in maintaining the stability and assembly of the basement membrane (BM). Its presence is essential for the structural integrity and functionality of the BM.
애플리케이션
The human placental collagen type IV (Sigma Aldrich 234154) is used:
- as a coating substrate for cell culture treated flasks to promote the growth and adhesion of CuFi-1 cells, providing reliable conditions for cell line propagation and maintenance
- to coat transwell inserts, providing a suitable substrate for the growth and formation of monolayers in intestinal organoid culture for trans epithelial electrical resistance (TEER) measurement and drug treatments
- to coat inserts for two-dimensional monolayer culture
생화학적/생리학적 작용
Collagen Type IV interacts with numerous binding partners. Type IV collagen forms a structural scaffold within the basement membrane (BM) along with other macromolecules like laminins, heparan sulfate proteoglycans, fibronectin, and entactin. This complex network regulates the interaction between the BM and adhering cells, providing critical support and functionality. Thereby, regulates cell adhesion and migration.
포장
Please refer to vial label for lot-specific concentration.
물리적 형태
In sterile 2 mM HCl.
기타 정보
Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3.
Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.
Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Toxicity: Standard Handling (A)
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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