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Merck

203389

Sigma-Aldrich

Blebbistatin, Racemic

≥97% (HPLC), liquid, Myosin II inhibitor, Calbiochem®

동의어(들):

InSolutionBlebbistatin, Racemic

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크기 선택


제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C18H16N2O2
Molecular Weight:
292.33
UNSPSC 코드:
12352200
NACRES:
NA.77
기술 서비스
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제품 이름

Blebbistatin, Racemic, InSolution, ≥97%, 50 mM in 90% DMSO, reversible inhibitor of nonmuscle myosin II

Quality Level

분석

≥97% (HPLC)

양식

liquid

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
desiccated (hygroscopic)
protect from light

배송 상태

wet ice

저장 온도

−20°C

일반 설명

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.

생화학적/생리학적 작용

Cell permeable: yes
Primary Target
ATPase
Product does not compete with ATP.
Reversible: yes
Target IC50: <100 µM inhibiting the ATPase and gliding motility of human platelets

포장

Packaged under inert gas

물리적 형태

A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203390 ) in 90% DMSO.

제조 메모

Following initial thaw, aliquot and freeze (-20°C).

기타 정보

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Flash Point (°C)

87 °C - closed cup - (Dimethylsulfoxide)


시험 성적서(COA)

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문서 라이브러리 방문

Kevin X Lau et al.
Nature communications, 11(1), 2420-2420 (2020-05-18)
Archetypal human pluripotent stem cells (hPSC) are widely considered to be equivalent in developmental status to mouse epiblast stem cells, which correspond to pluripotent cells at a late post-implantation stage of embryogenesis. Heterogeneity within hPSC cultures complicates this interspecies comparison.
Catarina Domingues et al.
Frontiers in cell and developmental biology, 8, 678-678 (2020-09-10)
The mechanical properties of the extracellular environment are interrogated by cells and integrated through mechanotransduction. Many cellular processes depend on actomyosin-dependent contractility, which is influenced by the microenvironment's stiffness. Here, we explored the influence of substrate stiffness on the proteome
Jung Hoon Cho et al.
Science advances, 8(38), eabq8486-eabq8486 (2022-09-24)
Primary cilia are specialized cell-surface organelles that mediate sensory perception and, in contrast to motile cilia and flagella, are thought to lack motility function. Here, we show that primary cilia in human and mouse pancreatic islets exhibit movement that is
Vivek K Gupta et al.
The Journal of cell biology, 220(8) (2021-06-17)
Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These morphological changes occur despite dividing cells being mechanically restricted by neighboring cells, indicating the need for extracellular force generation. Beyond driving cell

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