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Merck

17-10400

Sigma-Aldrich

Anti-phospho-MLKL (Ser358) Antibody Set

from rabbit, purified by affinity chromatography

동의어(들):

Mixed lineage kinase domain-like protein, MLKL

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

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기술 서비스
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기술 서비스
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도움 문의

생물학적 소스

rabbit

Quality Level

100

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human

기술

western blot: suitable

UniProt 수납 번호

Q8NB16

배송 상태

wet ice

타겟 번역 후 변형

phosphorylation (pSer358)

유전자 정보

human ... MLKL(197259)

일반 설명

Mixed lineage kinase domain-like protein (MLKL) is required for the execution of programmed necrosis. MLKL is a cell-permeable acrylamide compound that inhibits human, but not murine, MLKL adaptor function via covalent modification of Cys86. MLKL prevents the MLKL-RIP1-RIP3 necrosome complex from interacting with further downstream effectors.
This phospho-MLKL (Ser358) antibody set contains one vial of phospho-MLKL (Ser358) antibody and one vial of MLKL “Total” antibody.

RECOMMENDED USAGE:
It is recommended to first perform immunoprecipitation on your sample using the non-phosphorylated MLKL antibody for “total” MLKL protein isolation (see protocol section on page 2 of the Certificate of Analysis); then use the immunoprecipitated sample to detect the phosphorylated protein using the phospho-MLKL (Ser358) antibody via western blot.
~54 kDa

면역원

Epitope: Ser358
Linear peptide corresponding to the Protein Kinase Domain of phospho-MLKL (Ser358).

애플리케이션

Western Blot Analysis:
Untreated HT-29 cell lysates or lysates from HT-29 cells treated with Cycloheximide,Z-VAD-FMK and TNF alpha were first immunoprecipitated using the Anti-MLKL “total” antibody (1:1000 dilution) to isolate the MLKL protein.
The isolated “total” MLKL protein sample was then probed with Anti-phospho-MLKL (Ser358) (1:1000 dilution).
Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB.

생화학적/생리학적 작용

When used together and under the “Recommended Usage” instructions, this antibody set will detect MLKL phosphorylated at Ser358.

물리적 형태

100 µL of Anti-phospho-MLKL (Ser358) purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

100 µg (concentration: 1 mg/mL) of Anti- MLKL purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

분석 메모

Anti- MLKL (Total Antibody): Evaluated by Immunoprecipitation on HT-29 cell lysates.

Anti-phospho-MLKL (Ser358) Antibody: Evaluated by Dot Blot on phosphorylated and non-phosphorylated MLKL peptides.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
One vial of phospho-MLKL (Ser358) antibody

One vial of MLKL “Total” antibody

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Storage Class Code

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

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