Fractogel^® EMD COO^- (M)

8 x 100mm

MiniChrom Column Fractogel^® COO (M), 5 ml enables:

• Good resolution between monomers and aggregates
• Efficient aggregate and HCP removal combined with high yield
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD COO^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany