07-164
Anti-phospho-H2A.X (Ser139) Antibody
Upstate®, from rabbit
동의어(들):
H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, vertebrates
포장
antibody small pack of 25 μg
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
phosphorylation (pSer139)
유전자 정보
human ... HIST2H2AC(8338)
일반 설명
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
15 kDa
면역원
Epitope: a.a. 134-142
애플리케이션
Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
생화학적/생리학적 작용
Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.
물리적 형태
Format: Purified
제조 메모
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
분석 메모
Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE205
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Cells adapted to high NaCl have many DNA breaks and impaired DNA repair both in cell culture and in vivo.
Natalia I Dmitrieva, Qi Cai, Maurice B Burg
Proceedings of the National Academy of Sciences of the USA null
ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis.
Sanchez-Moran, E; Santos, JL; Jones, GH; Franklin, FC
Genes & Development null
High NaCl causes Mre11 to leave the nucleus, disrupting DNA damage signaling and repair.
Natalia I Dmitrieva, Dmitry V Bulavin, Maurice B Burg
American Journal of Physiology: Renal Physiology null
DNA replication is intrinsically hindered in terminally differentiated myotubes.
Pajalunga, D; Puggioni, EM; Mazzola, A; Leva, V; Montecucco, A; Crescenzi, M
Testing null
Absence of Wip1 partially rescues Atm deficiency phenotypes in mice.
Darlington, Y; Nguyen, TA; Moon, SH; Herron, A; Rao, P; Zhu, C; Lu, X; Donehower, LA
Oncogene null
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