생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
monoclonal
종 반응성
vertebrates (common species)
제조업체/상표
Upstate®
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG3
배송 상태
dry ice
타겟 번역 후 변형
unmodified
일반 설명
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Various
면역원
KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.
애플리케이션
Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.
Immunoprecipitation:
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
생화학적/생리학적 작용
Reactivity with other species has not been confirmed.
Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.
제조 메모
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
RIPA lysates from HEK293 cells.
RIPA lysates from HEK293 cells.
Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 07-221
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M.
Kobayashi, H; Nolan, A; Naveed, B; Hoshino, Y; Segal, LN; Fujita, Y; Rom, WN; Weiden, MD
Journal of immunology (Baltimore, Md. : 1950) (1950)
A rapid and efficient immunoenzymatic assay to detect receptor protein interactions: G protein-coupled receptors.
Zappelli, E; Daniele, S; Abbracchio, MP; Martini, C; Trincavelli, ML
International Journal of Molecular Sciences null
H Wu et al.
Neuroscience, 170(1), 149-155 (2010-07-06)
Previous studies have demonstrated that merlin acts as a tumor suppressor by blocking Ras-mediated signaling. However, the mechanism by which merlin controls cell proliferation has remained obscure. Here we show that merlin deficient tumors exhibited loss of p21, concomitant with
Verena Supper et al.
Journal of immunology (Baltimore, Md. : 1950), 196(3), 1387-1399 (2016-01-06)
The Ig superfamily member CD147 is upregulated following T cell activation and was shown to serve as a negative regulator of T cell proliferation. Thus, Abs targeting CD147 are being tested as new treatment strategies for cancer and autoimmune diseases.
Patrick Guye et al.
Nature communications, 7, 10243-10243 (2016-01-07)
Human induced pluripotent stem cells (hiPSCs) have potential for personalized and regenerative medicine. While most of the methods using these cells have focused on deriving homogenous populations of specialized cells, there has been modest success in producing hiPSC-derived organotypic tissues
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