E. coli Extract Polar

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

E. coli Extract Polar has been used for the preparation of lipid standards for validation purposes. It has also been used for determining the molar percentages of lipids.

E. coli Extract Polar has been used:

• in liposome preparation for spectroscopy studies using fluorescent membrane dye, laurdan
• in multilamellar vesicles generation
• as standard lipid material for tandem mass spectrometry analysis

E. coli Extract Polar is suitable: to prepare lipid standard material by dissolving in methanol to prepare small unilamellar vesicle to prepare liposomes (in Tris-hydrochloric acid (HCl)

E. coli Polar lipid extracts are useful for reconstituting membrane proteins. The phosphatidylethanolamine is the predominant lipid component of E. coli extract polar extract. The minor components include the phosphatidylglycerol (PG) and cardiolipin. These lipids are majorly derived from the leaflets of cytoplasmic membrane and inner leaflet of outer membrane. E. coli polar lipid extracts form bilayer by vesicle fusion.

5 mL Clear Glass Sealed Ampule (100600P-100mg)

5 mL Clear Glass Sealed Ampule (100600P-500mg)

60 mL Amber Wide Mouth Screw Cap Glass Bottle (100600P-1g)

Avanti Research is a trademark of Avanti Polar Lipids, LLC