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50994-U

BIOshell™ Glycan (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
15 cm × 2.1 mm
Particle size:
2.7 μm
Matrix active group:
hydroxy, penta- phase
Pore size:
90 Å
Matrix:
spherical silica particle platform, superficially porous particle
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Product Name

BIOshell™ Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size

material

stainless steel hardware (column)

Quality Level

100

agency

suitable for USP L86

description

shell thickness (0.5 μm), solid core (1.7 μm)

product line

BIOshell™

feature

endcapped: no

analyte chemical class(es)

glycans

manufacturer/tradename

BIOshell™

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi), 1000 bar pressure (14500 psi), 60 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

135 m2/g

matrix

spherical silica particle platform, superficially porous particle

matrix active group

hydroxy, penta- phase

particle size

2.7 μm

pore size

90 Å

operating pH range

2-9

separation technique

hydrophilic interaction (HILIC)

General description

BIOshell™ Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. The Fused-Core columns provide improved resolution when compared to conventional LC methods thus allowing more reliable quantitation and confident identification of glycans.

View the BIOshell Glycan HPLC Columns Flyer

Application

BIOshell™ Glycan HPLC Columns are high-performance liquid chromatography columns with Fused-Core particle design. Their column chemistry is suitable for hydrophilic interactive liquid chromatography (HILIC) analysis of biomolecules like oligosaccharides.

Features and Benefits

BIOshell™ Glycan HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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Protocols

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

Articles

BIOshell™ Glycan U/HPLC Columns

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Released N-Glycan Analysis of a Therapeutic Antibody Using BIOshell™ Glycan Column

This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.

View All Articles

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Global Trade Item Number

SKUGTIN
50994-U04061833363614