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Merck

WGA3

Sigma-Aldrich

GenomePlex® WGA Reamplification Kit

Reamplification of WGA product with minimal bias

Synonym(s):

GenomePlex® whole genome amplification reamplification kit, Whole genome reamplification kit

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

Key Documents

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Quality Level

200

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

GenomePlex® WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It allows the user to obtain even higher yields of desired DNA without disturbing the original, limited source of genomic DNA. The genomic DNA can be reamplified up to five cycles with minimal allele dropout producing an even higher yield. It provides the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Application

GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP. It has also been used in the reamplification of WGA2-amplified genomic DNA to generate >10 μg gDNA for microarray hybridization.

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit Components Only

Product No.
Description
  • Deoxynucleotide Mix, 10 mM

Kit Components Also Available Separately

Product No.
Description
SDS
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mLSDS

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK6883
SLCC6261
SLBZ9799
SLBZ9798
SLBS6112

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Churamani Khanal et al.
Plant disease, 103(8), 1835-1842 (2019-06-14)
This study employed single nucleotide polymorphisms (SNPs) to determine the genetic variability present in 26 isolates of Rotylenchulus reniformis from Louisiana, Mississippi, Arkansas, South Carolina, Georgia, Hawaii, and Alabama. Genomic DNA from reniform nematode was extracted and increased quantitatively using
Aleksey Komissarov et al.
Genes, 9(10) (2018-09-23)
The Asian seabass (Lates calcarifer) is a bony fish from the Latidae family, which is widely distributed in the tropical Indo-West Pacific region. The karyotype of the Asian seabass contains 24 pairs of A chromosomes and a variable number of
Mirella L Meyer-Ficca et al.
Chromosoma, 122(4), 319-335 (2013-06-05)
The mammalian sperm nucleus is characterized by unique properties that are important for fertilization. Sperm DNA retains only small numbers of histones in distinct positions, and the majority of the genome is protamine associated, which allows for extreme condensation and
Nerea Paz et al.
PloS one, 8(4), e60238-e60238 (2013-04-09)
Chromosome territories constitute the most conspicuous feature of nuclear architecture, and they exhibit non-random distribution patterns in the interphase nucleus. We observed that in cell nuclei from humans with Down Syndrome two chromosomes 21 frequently localize proximal to one another
Atsuo Yoshido et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 21(2), 149-164 (2013-03-22)
Our previous studies revealed a considerably high level of chromosomal polymorphism in wild silkmoths, Samia cynthia ssp. (Lepidoptera: Saturniidae). Geographical populations of this species complex differ in chromosome numbers and show derived sex chromosome systems including Z0/ZZ in S. cynthia
View All Related Papers

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Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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Our commitment to the Life Sciences continues through significant advances in PCR technology. Improvements to the key parameters of yield, length, specificity, and fidelity, as well as new applications for nucleic acid amplification form the basis of our research and development focus. We are dedicated to providing you with the newest technology as the field of Nucleic Acid Amplification evolves, making Sigma your preferred supplier for PCR products.

GenomePlex Whole Genome Amplification
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