Skip to Content
Merck

TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

Synonym(s):

mitochondrial activity assay

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

 kit sufficient for 1,000 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

Related Categories

General description

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Biochem/physiol Actions

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

Signal Word

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCL3638
SLCJ1228
SLCG9357
SLCG8036
SLCF2808

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Human renal organic anion transporter 1-dependent uptake and toxicity of mercuric-thiol conjugates in Madin-Darby canine kidney cells
Aslamkhan AG, et al.
Molecular Pharmacology, 63(3), 590-596 (2003)
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Baumgart J, et al.
Biomaterials, 33(7), 2345-2350 (2012)
Anumol Shashikumar et al.
Cytotechnology, 65(2), 253-262 (2012-07-26)
Testicular cell culture of crab, Scylla serrata (Forskal) was used to study the effects of White spot syndrome virus (WSSV). We are showing the susceptibility of cell culture of crabs to WSSV. The proliferating cell culture of testes were maintained
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
Weifeng Wang et al.
Journal of virology, 91(9) (2017-02-17)
The human immunodeficiency virus type 1 (HIV-1) capsid protein is an attractive therapeutic target, owing to its multifunctionality in virus replication and the high fitness cost of amino acid substitutions in capsids to HIV-1 infectivity. To date, small-molecule inhibitors have
View All Related Papers

Articles

세포 생존력 및 증식 분석

암, 신경과학 및 줄기세포 연구에 응용할 수 있는 세포 증식(BrdU, MTT, WST1), 세포 생존력 및 세포 독성 실험을 위한 세포 기반 분석입니다.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service