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T3069

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700
Technical Service
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Quality Level

200

grade

BioPerformance Certified, Molecular Biology

form

solution

concentration

2 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

color

colorless

pH

7.95—8.05, 8.0

useful pH range

7.0-9.0

density

1.07—1.09  g/cm3

absorption

≤0.05 at 290 at 40%

General description

Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Lot/Batch Number
117K84081
117K8408
046K8417
042K8411

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(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Optimal DNA extractions from blood on preservation paper limits conservation genomic but not conservation genetic applications
Stowell SML, et al.
Journal for nature conservation, 46 (46)
Universal buffers for use in biochemistry and biophysical experiments
Brooke D, et al.
AIMS Biophysics, 2(3) (2)
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Global Trade Item Number

SKUGTIN
T3069-100ML04061838175762
T3069-1L04061838260215