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SAB4200689

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

Name: Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal
Brand: SIGMA

clone 5C5, hybridoma cell culture supernatant

Synonym(s):

Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

5C5, monoclonal

Species reactivity:

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

Application:

ELISA, IF, IHC

Citations:

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Properties

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5C5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

packaging

antibody small pack of 25 μL

technique(s)

ELISA: suitable, immunoblotting: 1:500-1:1000 using rat heart tissue extracts, immunofluorescence: 1:500-1000 using human HeLa cells., immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin^®-Peroxidase staining system.

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)

Description

General description

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Application

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:

indirect immunofluorescence staining
immunohistochemistry
enzyme-linked immunosorbent assay (ELISA)
immunoblotting
immunofluorescence

Biochem/physiol Actions

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c