The GlycoProfile^™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosylphosphatidylinositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.

Application

GlycoProfile^™ 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and human epididymis protein 4. It has also been used for labelling free oligosaccharides from mouse spleen.

While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.

Legal Information

Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.

Description

2-AB Labeling Reagent 2 x 5

Dimethyl sulfoxide (DMSO) 2 x 350

Acetic acid, glacial 2 x 200

Reductant (sodium cyanoborohydride) 2 x 6

Pictograms

GHS02,GHS06,GHS05,GHS09

Signal Word

Danger

Hazard Statements

H228,H260,H300 + H310 + H330,H314,H410

Precautionary Statements

P210 - P231 + P232 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Sol. 1 - Skin Corr. 1A - Water-react 1

Supplementary Hazards

EUH032

Storage Class Code

4.3 - Hazardous materials which set free flammable gases upon contact with water

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Separation of 2-aminobenzamide labeled glycans using hydrophilic interaction chromatography columns packed with 1.7 mum sorbent

Ahn J, et al.

Journal of Chromatography. B, Biomedical Sciences and Applications, 878(3-4), 403-408 (2010)

Expression and biochemical characterization of recombinant human epididymis protein 4

Hua L, et al.

Protein Expression and Purification, 102, 52-62 (2014)

Enhancing the antiviral potency of ER alpha-glucosidase inhibitor IHVR-19029 against hemorrhagic fever viruses in vitro and in vivo

Ma J, et al.

Antiviral research, 150, 112-122 (2018)

Production of α2,6-sialylated IgG1 in CHO cells.

Céline Raymond et al.

mAbs, 7(3), 571-583 (2015-04-16)

The presence of α2,6-sialic acids on the Fc N-glycan provides anti-inflammatory properties to the IgGs through a mechanism that remains unclear. Fc-sialylated IgGs are rare in humans as well as in industrial host cell lines such as Chinese hamster ovary

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