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P9577

PCR 50 - 2,000 bp Marker

Name: PCR 50 - 2,000 bp Marker
Brand: SIGMA

for electrophoresis of PCR fragments

Synonym(s):

50-2000 bp ladder, PCR ladder for agarose gel electrophoresis, PCR marker for agarose gel electrophoresis, RTU PCR DNA ladder, RTU PCR DNA marker, ready to use DNA ladder, ready to use DNA marker

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105337

Form:

liquid

Storage temp.:

−20°C

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Properties

Quality Level

200

form

liquid

usage

sufficient for 50 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Description

General description

Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.

Other Notes

Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:

1 vial (250 μl volume) PCR Marker
1 vial 6× loading buffer (250 μl volume) for diluting samples

Storage Class

10 - Combustible liquids

ppe

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Protocols

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Global Trade Item Number

SKU	GTIN
P9577-1VL	04061835573882