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Merck

P2980

Sigma-Aldrich

Pertussis toxin

from Bordetella pertussis, buffered aqueous glycerol solution, protein endotoxin

Synonym(s):

Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen

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About This Item

CAS Number:
70323-44-3
MDL number:
MFCD00131841
UNSPSC Code:
12352200
NACRES:
NA.77

Key Documents

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Product Name

Pertussis toxin from Bordetella pertussis, buffered aqueous glycerol solution

description

aseptically packaged

Quality Level

200

form

buffered aqueous glycerol solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC

InChI

1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3

InChI key

WOPKHAQDUMDJIY-UHFFFAOYSA-N

Gene Information

Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)

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General description

Pertussis (Pertussis) toxin (PTX) is a protein isolated from the bacterium Bordatella (Bordatella) coccobaccillus. The toxin is excreted in a non-active state. When the B oligomer of the bacterium, Bordatella coccobacillus, comes in contact with the cell membrane, an S1 subunit of its A protomer is activated. This activation may be mediated by glutathione and/or ATP.

Application

Inactive toxin form released from B. pertussis.
Pertussis toxin from Bordetella pertussis has been used: to enhance cardiac myosin immunization of rats to induce myocarditis; to break down the blood–brain barrier in the Cuprizone and cuprizone/MOG-EAE model; as an inhibitor of Gαi signaling
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCP9354
MKCG2371
MKCP6226
MKCP6227
MKCN3229

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Antonietta Franco et al.
Journal of molecular and cellular cardiology, 121, 60-68 (2018-07-04)
G-protein receptor kinases (GRKs) regulate adult hearts by modulating inotropic, chronotropic and hypertrophic signaling of 7-transmembrane spanning neurohormone receptors. GRK-mediated desensitization and downregulation of β-adrenergic receptors has been implicated in adult heart failure; GRKs are therefore a promising therapeutic target.
Su Jin Hwang et al.
Laboratory investigation; a journal of technical methods and pathology, 90(1), 9-19 (2009-10-14)
FTY720, a sphingosine 1-phosphate (S1P) receptor modulator, suppresses immune responses by inhibiting T-cell migration into target tissues; however, it does not alter T-cell functions. In this study, we investigated the biological effects of FTY720 on NKT cells. Unlike T cells
Jianghong Zhong et al.
Journal of neuroinflammation, 17(1), 113-113 (2020-04-12)
Increasing evidence has suggested that a single nucleotide polymorphism in the Ncf1 gene is associated with experimental autoimmune encephalomyelitis (EAE). However, the mechanisms of NCF1-induced immunoregulatory effects remain poorly understood. In this study, we focus on NCF1 deficiency-mediated effects on
Jason M Conley et al.
The Journal of biological chemistry, 297(1), 100881-100881 (2021-06-19)
GPR17 is a G-protein-coupled receptor (GPCR) implicated in the regulation of glucose metabolism and energy homeostasis. Such evidence is primarily drawn from mouse knockout studies and suggests GPR17 as a potential novel therapeutic target for the treatment of metabolic diseases.
Jonathan Hoggatt et al.
Cell, 172(1-2), 191-204 (2017-12-12)
Hematopoietic stem cell transplantation is a potential curative therapy for malignant and nonmalignant diseases. Improving the efficiency of stem cell collection and the quality of the cells acquired can broaden the donor pool and improve patient outcomes. We developed a
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