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G7396

β-Glucuronidase from Escherichia coli

Name: β-Glucuronidase from <I>Escherichia coli</I>
Brand: SIGMA

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

MFCD00199013

EC Number:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

1,000,000-5,000,000 units/g protein (30 min assay)

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Properties

type

Type IX-A

Quality Level

300

form

lyophilized powder

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

mol wt

290 kDa

composition

Protein, 30-70% biuret

shipped in

wet ice

storage temp.

−20°C

Description

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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pictograms

GHS08

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

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Certificates of Analysis (COA)

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Protocols

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Global Trade Item Number

SKU	GTIN
G7396-1MU	04061835557035
G7396-25KU	04061833642702
G7396-2MU	04061833642719
G7396-500KU	04061833642726
G7396-125KU	04061835557028
G7396-250KU	04061835557042