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Merck

F6057

Sigma-Aldrich

Fluoroshield™ with DAPI

liquid

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

Key Documents

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Product Name

Fluoroshield™ with DAPI, liquid

description

histology mounting medium

Quality Level

200

form

liquid

composition

Sodium Azide, ≤1% (also contains other ingredients)
Tris-HCl

technique(s)

immunofluorescence: suitable

pH range

7.9-8.3

solubility

water: soluble

fluorescence

λex 360 nm; λem 460 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Related Categories

General description

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Application

Fluoroshield™ with DAPI has been used:
  • as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
  • as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
  • as mounting solution for immunostained heart sections for nuclei detection

Legal Information

FluoroShield is a trademark of ImmunoBioScience Corp.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM1438
SLCK9073
SLCJ8103
SLCG8078
SLCG2144

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Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
Maria L Spletter et al.
eLife, 7 (2018-05-31)
Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group
M Herbette et al.
DNA repair, 57, 139-150 (2017-08-07)
Maintaining the integrity of genetic information across generations is essential for both cell survival and reproduction, and requires the timely repair of DNA damage. Histone-modifying enzymes play a central role in the DNA repair process through the deposition and removal
Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Nascimento DS, et al.
Stem Cell Research & Therapy, 5(1), 5-5 (2014)
S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
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