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Merck

F0926

Sigma-Aldrich

Fetal Bovine Serum

USDA approved, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Bovine Sera, Fetal Calf Sera, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71
Biological source:
bovine fetus
Sterility:
sterile-filtered
Shipped in:
dry ice

Key Documents

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

quality

USDA approved

composition

Hemoglobin, ≤25 mg/dL

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.

Application

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Fetal Bovine Serum has been used for the following cell cultures:
  • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components).
  • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).
  • Isolation and 3D culture of primary mammary epithelial organoids
  • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors)
USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
9C0079
22M275
22M276
22E322
21K470

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Carmen N Hernández-Candia et al.
Methods (San Diego, Calif.), 164-165, 81-90 (2019-03-25)
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PloS one, 9(2), e88893-e88893 (2014-03-04)
Use of fusion protein tags to investigate lysosomal proteins can be complicated by the acidic, protease-rich environment of the lysosome. Potential artifacts include degradation or release of the tag and acid quenching of fluorescence. Tagging can also affect protein folding
Kevin Anton et al.
PloS one, 7(4), e35036-e35036 (2012-04-13)
Mesenchymal stem cells (MSCs) exhibit tropism for sites of tissue injury and tumors. However, the influence of the microenvironment on MSC phenotype and localization remains incompletely characterized. In this study, we begin to define a macrophage-induced MSC phenotype. These MSCs
Weiran Shen et al.
Journal of virology, 89(19), 10097-10109 (2015-08-01)
Human bocavirus 1 (HBoV1) is a single-stranded DNA parvovirus that causes lower respiratory tract infections in young children worldwide. In this study, we identified novel splice acceptor and donor sites, namely, A1' and D1', in the large nonstructural protein (NS1)-encoding
Jonathan T Lei et al.
Cell reports, 24(6), 1434-1444 (2018-08-09)
RNA sequencing (RNA-seq) detects estrogen receptor alpha gene (ESR1) fusion transcripts in estrogen receptor-positive (ER+) breast cancer, but their role in disease pathogenesis remains unclear. We examined multiple ESR1 fusions and found that two, both identified in advanced endocrine treatment-resistant
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성공으로 이끄는 우태혈청.

머크의 포괄적인 우태혈청(FBS) 포트폴리오에서 귀하의 실험실 요건과 예산에 적합한 제품을 찾아보십시오. 지구촌의 문제가 FBS 가격에 미치는 영향에 대해 알아보십시오.

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