Skip to Content
Merck

E5134

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

Synonym(s):

Sequestrene Na2, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
6381-92-6
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
205-358-3
UNSPSC Code:
12352107
PubChem Substance ID:
24894605
NACRES:
NA.31

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

Quality Level

300

Assay

99.0-101.0% (titration)

form

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%
Pb: ≤0.002%

suitability

suitable for electrophoresis
suitable for molecular biology

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
  • an anticoagulant for whole blood collection
  • a stabilizer solution for freezing cell culture samples
  • in combination with PBS and trypsin, to trypsinize cells
  • for phytoextraction of heavy metals in polluted soils

Features and Benefits

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Warning

Hazard Statements

H332 - H373

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
CDBF8329
SLCL6568
SLCL6563
SLCK9139
SLCK9010

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma.
Dalley AJ, et al.
Cancer Medicine, 3(2), 273-283 (2014)
External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
Erwin G Zoetendal et al.
Nature protocols, 1(2), 870-873 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that develops in time and space. The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA.
Michi Miura et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 28(3-4), 381-393 (2020-10-19)
CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in
View All Related Papers

Protocols

mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 PAGE/SDS-PAGE용 핸드캐스팅 겔

폴리아크릴아미드 겔 화학의 개요와 mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 폴리아크릴아미드 겔 핸드캐스팅에 대한 상세 지침

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Protein Interactions

Formaldehyde cross-linking protocol detects specific protein interactions with confidence.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service