DCAS9P300
Sigma CRISPR dCas9-p300 Activator Expression Plasmid
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About This Item
recombinant
expressed in E. coli
Quality Level
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Application
- Functional Genomics/Target ValidationEpigenetic Modification
- Transcriptional Activation
Features and Benefits
- The Sigma CRISPR dCas9p300 plasmid co-expresses p300-HAT and GFP, to easily monitor delivery and expression in your target cell type (figure 1)gRNAs can successfully direct nuclease-deficient Cas9 (dCas9) fused to p300 HAT catalytic domain to increase levels of histone acetylation and endogenous gene expressionThe dCas9-p300 histone acetylation approach represents a distinct mechanism of action relative to dCas9-VP64 or other similar gene activation motifs
Other Notes
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
Related Content
Sigma-Aldrich® Advanced Genomics는 CRISPR, Cas9, 합성 가이드 RNA(sgRNA) 및 ZFN(Zinc Finger Nuclease)을 포함한 유전자 편집 및 침묵 기술의 선도적인 제공업체입니다.
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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