Key Documents

View All Documentation

D6421

DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

Select a Size

Change View

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO₃: yes
HEPES: 15 mM
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.

Application

DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Suitable for growth of wide range of cell types in low serum conditions.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.

Preparation Note

Supplement with 0.365 g/L L-glutamine.

Still not finding the right product?

Explore all of our products under Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

유도만능줄기세포 배양 프로토콜

iPS 세포 해동, 증식, 냉동, 특성화를 포함한 인간 유도만능줄기세포(iPSC)를 위한 줄기세포 배양의 단계별 프로토콜.

Articles

Nutrient Mixture F-12 Ham Formulation

Global Trade Item Number

SKU	GTIN
D6421-24X500ML	04061833587515
D6421-500ML	04061833587522
D6421-6X500ML	04061833587539