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C9801

Casein, N,N-dimethylated from bovine milk

Name: Casein, N,N-dimethylated from bovine milk
Brand: SIGMA

essentially salt-free, lyophilized powder

Synonym(s):

N,N-dimethylcasein

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About This Item

CAS Number:

9000-71-9

UNSPSC Code:

12352202

NACRES:

NA.61

MDL number:

MFCD00130742

Form:

essentially salt-free, lyophilized powder

Assay:

≥90% (colorimetric)

Biological source:

bovine milk

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Properties

biological source

bovine milk

Quality Level

200

assay

≥90% (colorimetric)

form

essentially salt-free, lyophilized powder

technique(s)

activity assay: suitable

UniProt accession no.

P02662, P02663, P02666, P02668

storage temp.

−20°C

SMILES string

[P](=O)(OCC(NC(=O)C(NC(=O)C(N)Cc1ccccc1)CCC(=O)N)C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(C(O)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)N)C(=O)NC(CC(=O)O)C(=O)NC(C

InChI

1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)

InChI key

BECPQYXYKAMYBN-UHFFFAOYSA-N

Gene Information

bovine ... CSN1S1(282208), CSN1S2(282209), CSN2(281099), CSN3(281728)

Description

General description

Casein is a milk protein, that is not expensive and nontoxic. highly stable. This phosphoproteins accounts for 80% of the total protein present in bovine milk. Casein is a member of one of the larger family of secretory calcium?binding phosphoproteins.

Application

Casein, N,N-dimethylated from bovine milk can be used in assays to detect and help determine the functional role of tissue transglutaminase (tTG) in disease. In particular, Casein, N,N-dimethylated has been used in the mechanic and kinetic analysis of tTG on protein substrates, which may be linked to neurodegenerative disease.

This preparation has less than 10% reactivity with 2,4,6-trinitrobenzenesulfonic acid (TNBS) compared to non-methylated casein.

Protease substrate that has been used to assay basilase and a subtilisin analog that had been produced by site-directed mutagenesis.

Casein, N,N-dimethylated from bovine milk has been used:

in the determination of general proteolytic activity
as a substrate to study the effect of dephosphorylation on the calcium-activated neutral proteases (CANP) proteolysis of paired helical filaments (PHF) II-τ
in transglutaminase (TG) assay

Biochem/physiol Actions

Casein is highly preferred as a drug delivery vehicle. It possess antioxidant activity.

Preparation Note

Reductive methylation of C5890 by the method of Cabacungan, J.C., et al., Anal. Biochem., 124, 272 (1982).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Immunoassay of in vitro activated human tissue transglutaminase.

Johannes Wolf et al.

Analytical biochemistry, 411(1), 10-15 (2010-12-15)

Tissue transglutaminase (tTG) is a calcium-dependent enzyme that exerts a variety of physiological functions and is involved in various pathoprocesses. To characterize the role of tTG in disease, simple assays are necessary for detection. We developed a highly sensitive enzyme-linked

A highly active and oxidation-resistant subtilisin-like enzyme produced by a combination of site-directed mutagenesis and chemical modification.

H Grøn et al.

European journal of biochemistry, 194(3), 897-901 (1990-12-27)

The subtilisins are known to be susceptible to chemical oxidation due to the conversion of Met222 into the corresponding sulfoxide. A number of derivatives with resistance towards oxidation have previously been prepared by replacement of this group with the other

Characterization of recombinant camel chymosin reveals superior properties for the coagulation of bovine and camel milk

Kappeler SR, et al.

Biochemical and biophysical research communications, 342(2), 647-654 (2006)

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Global Trade Item Number

SKU	GTIN
C9801-50G	04061833531204
C9801-5G	04061833531211
C9801-1G	04061833531181
C9801-25G	04061833531198

Key Documents

Casein, N,N-dimethylated from bovine milk

essentially salt-free, lyophilized powder

Select a Size

About This Item

biological source

Quality Level

assay

form

technique(s)

UniProt accession no.

storage temp.

SMILES string

InChI

InChI key

Gene Information

General description

Application

Biochem/physiol Actions

Preparation Note

Safety Information

Storage Class

wgk

flash_point_f

flash_point_c

ppe

Documentation

Certificates of Analysis (COA)

Already Own This Product?

Peer Reviewed Papers

Global Trade Item Number