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Merck

C1655

Sigma-Aldrich

Cholera Toxin B subunit

GM1-binding, lyophilized powder

Synonym(s):

CTB

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About This Item

MDL number:
MFCD02356522
UNSPSC Code:
12352200
PubChem Substance ID:
329774902
NACRES:
NA.77

Key Documents

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Product Name

Cholera Toxin B subunit, FITC conjugate, lyophilized powder

conjugate

FITC conjugate

Quality Level

200

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~20% Lowry

extent of labeling

~1.0 mol FITC per mol protein

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

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Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
  • to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat
  • to stain epididymal sperms of mice
  • to stain peritoneal macrophages of mice

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Analysis Note

Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H332,H412

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
109M4073V
049M4142V
109M4072V
11190214
088M4101V

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Emily Igel et al.
The Journal of biological chemistry, 297(3), 101106-101106 (2021-08-24)
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Almut Dufner et al.
Cell communication and signaling : CCS, 9(1), 6-6 (2011-03-15)
The B cell antigen receptor (BCR) and pathogen recognition receptors, such as Toll-like receptor 4 (TLR4), act in concert to control adaptive B cell responses. However, little is known about the signaling pathways that integrate BCR activation with intrinsic TLR4
Birgit Bauer et al.
FEBS letters, 541(1-3), 155-162 (2003-04-23)
Protein kinase (PK) Ctheta and Akt/PKBalpha cooperate in T cell receptor/CD28-induced T cell signaling. We here demonstrate the recruitment of endogenous Akt1 and PKCtheta to lipid rafts in CD3-stimulated T cells. Further we show that Myr-PKCtheta mediates translocation of endogenous
Laura Aragoneses-Fenoll et al.
Journal of leukocyte biology (2021-02-03)
The interaction between the T-lymphocyte costimulatory molecule ICOS and its ligand (ICOS-L) is needed for efficient immune responses, but expression levels are tightly controlled, as altered expression of ICOS or ICOS-L may lead to immunodeficiency, or favor autoimmune diseases and
Jill M Steinbach et al.
Acta biomaterialia, 30, 49-61 (2015-11-26)
The surface modification of nanoparticles (NPs) can enhance the intracellular delivery of drugs, proteins, and genetic agents. Here we studied the effect of different surface ligands, including cell penetrating peptides (CPPs), on the cell binding and internalization of poly(lactic-co-glycolic) (PLGA)
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