B6916
Bradford Reagent
for 0.1-1.4 mg/ml protein
Synonym(s):
Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent
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General description
Application
Features and Benefits
- The reagent is ready to use. No mixing or dilution required.
- Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
- Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
- Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
- Can be used in microwell plate assays.
- Inexpensive assay.
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
Target Organs
Eyes,Central nervous system
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Protocols
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
Related Content
We have developed a novel, high-binding capacity, antibodybased resin for the depletion of twenty high abundance proteins in human plasma. The technology is a significant improvement over existing kits that remove 2, 6, or 12 proteins.
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
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