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70990

α-Cyano-4-hydroxycinnamic acid

suitable for matrix substance for MALDI-MS, ≥99.0% (HPLC)

Synonym(s):

α-CCA, α-CHCA, α-Cyano, 4-HCCA, ACCA

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About This Item

Linear Formula:
HOC6H4CH=C(CN)CO2H
CAS Number:
28166-41-8
Molecular Weight:
189.17
UNSPSC Code:
41115710
NACRES:
NA.21
PubChem Substance ID:
329762519
EC Number:
248-879-1
Beilstein/REAXYS Number:
3271427
MDL number:
MFCD00004204
Assay:
≥99.0% (HPLC)
Form:
solid
Quality level:
100
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Quality Level

100

assay

≥99.0% (HPLC)

form

solid

analyte chemical class(es)

glycans, peptides, proteins

technique(s)

MALDI-MS: suitable

mp

245-250 °C

solubility

methanol: 10 mg/mL, clear

cation traces

Ba: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, K: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤10 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

absorption

≥2000 at 337 nm (mol. absorption)

suitability

suitable for matrix substance for MALDI-MS

SMILES string

OC(=O)\C(=C\c1ccc(O)cc1)C#N

InChI

1S/C10H7NO3/c11-6-8(10(13)14)5-7-1-3-9(12)4-2-7/h1-5,12H,(H,13,14)/b8-5+

InChI key

AFVLVVWMAFSXCK-VMPITWQZSA-N

General description

α-Cyano-4-hydroxycinnamic acid (α-CHCA) is widely used as a matrix in order to facilitate ionization of peptides and proteins in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer.

Application

α-CHCA was used as matrix in improving the detection of low concentration protein digests on MALDI-TOF.

Other Notes

Matrix substoncitatioance used in MALDI-MS (matrix-assisted laser desorption ionization MS) peptide analysis in proteomics.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1B

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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S.D. Patterson, V. Katta
Analytical Chemistry, 66, 3729-3729 (1994)
J Gobom et al.
Analytical chemistry, 73(3), 434-438 (2001-02-24)
We present a new MALD1 sample preparation technique for peptide analysis using the matrix alpha-cyano-4-hydroxy-cinnamic acid (CHCA) and prestructured sample supports. The preparation integrates sample purification, based on the affinity of microcrystalline CHCA for peptides, thereby simplifying the analysis of
Xiangping Zhu et al.
Journal of biomolecular techniques : JBT, 14(4), 298-307 (2004-01-13)
Alpha-cyano-4-hydroxycinnamic acid (alpha-CHCA) as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer. The matrix itself also ionizes and so do its sodium and potassium adducts. Matrix clusters and metal
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Global Trade Item Number

SKUGTIN
70990-1G-F04061832843384
70990-250MG-F04061832843513