UFC9030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
Synonym(s):
15 mL sample volume, 30 kDa MWCO, Centrifugal concentrator
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About This Item
Quality Level
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL , 15 mL
pore size
30 kDa MWCO
greener alternative category
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Legal Information
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Protocols
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
면역글로불린을 위한 정제 프로토콜에는 단백질 A 또는 G 크로마토그래피 배지에 대한 친화성 결합, 비결합 단백질 세척, 낮은 pH 버퍼를 이용한 용리가 포함됩니다.
Articles
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.
Related Content
다운스트림 분석을 위한 정제 방식은 시료의 복잡성을 줄이고 간섭을 제거하며 분석물을 농축하여 보다 정확한 결과를 얻을 수 있습니다.
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| UFC903096 | 04053252400131 |
| UFC903008 | 04053252579066 |
| UFC903024 | 04053252724732 |