UFC8030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 30 kDa
Synonym(s):
Centrifugal concentrator,30 kDa MWCO, 4 mL sample volume
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About This Item
Quality Level
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
4 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL , 4 mL
pore size
30 kDa MWCO
greener alternative category
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Legal Information
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Storage Class
11 - Combustible Solids
Certificates of Analysis (COA)
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Protocols
Biomarkers play an essential role in the drug discovery and development process.
The human plasma proteome is a vast resource for biomarker discovery and investigation.
인간 혈장 단백질체는 바이오마커 발견 및 연구를 위한 막대한 자원입니다.
Articles
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| UFC803024 | 04053252725579 |
| UFC803008 | 04053252617263 |
| UFC803096 | 04053252502057 |