UFC201024
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa
Synonym(s):
Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume
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About This Item
Quality Level
material
polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
2 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
volume
2 mL
working volume
≤2 mL , 2 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
General description
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
- to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
- Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
- Desalting, buffer exchange, or diafiltration
Features and Benefits
- Amicon® Ultra 2ml 10K 24PK
- Concentrates 2 mL down to 15-70 μl
- 98% sample recovery
- Fast processing time - 10 to 30 minute spin times
- Engineered dead stop
- The reverse spin capability provides consistent recoveries
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
Other Notes
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Protocols
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Articles
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
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"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange the buffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in an Amicon® Ultra centrifugal device."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| UFC201024 | 04053252691782 |