SCR004
Alkaline Phosphatase Detection Kit
This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
Synonym(s):
ALP Stain, Alkaline Phosphtase Stem Cell Staining Kit, Stem Cell AP Staining Kit
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About This Item
Quality Level
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
shipped in
wet ice
General description
Application
Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].
For Research Use Only; Not for use in diagnostic procedures
Stem Cell Research
Preparation Note
When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
Other Notes
Napthol AS-BI phosphate solution (2mg/mL) in a buffer, pH 8.5 (Part No. CS235583). Two bottles.
Legal Information
Disclaimer
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling
Protocols
iPS 세포 해동, 증식, 냉동, 특성화를 포함한 인간 유도만능줄기세포(iPSC)를 위한 줄기세포 배양의 단계별 프로토콜.
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
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As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
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