SCGP00525
Millipore® Steriflip® Vacuum Tube Top Filter
pore size 0.22 μm, polyethersulfone membrane, funnel capacity 50 mL, pack of 25 ea
Synonym(s):
Steriflip-GP Sterile Centrifuge Tube Top Filter Unit, tube top sterile filter unit, tube top vacuum filter, tube top vacuum sterile filtering unit
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About This Item
material
acrylic housing
polyethersulfone membrane
polypropylene
Quality Level
sterility
sterile
product line
Steriflip®
feature
holdup volume 0.6 mL
hydrophilic
packaging
pack of 25 ea
parameter
45 °C max. temp.
technique(s)
sterile filtration: suitable
H
138 mm
diam.
6.4 cm
filter diam.
40 mm
filtration area
7 cm2
funnel capacity
50 mL
working volume
50 mL
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
double lead thread inlet (with vacuum port)
double lead thread outlet for 50 mL centrifuge tube
shipped in
ambient
General description
This Steriflip® 0.22 μm pore size polyethersulfone (PES) membrane filter unit combines a 50 mL centrifuge tube with a cap for processing and storage.
Application
Steriflip® filters are designed to be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
- Eliminates messy sample transfer via a simple vacuum-assisted, closed system
- Can isolate cells from cellular debris using any of the various pore-sized nylon net filters
- Ideal for sterilizing small batches of culture media
- Attaches to any standard 50 mL centrifuge tube, eliminating unnecessary materials, time and risk of spillage
Legal Information
Certificates of Analysis (COA)
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Articles
Select the right sterile filter by media, volume, and application.
Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.
Protocols
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
면역글로불린을 위한 정제 프로토콜에는 단백질 A 또는 G 크로마토그래피 배지에 대한 친화성 결합, 비결합 단백질 세척, 낮은 pH 버퍼를 이용한 용리가 포함됩니다.
Related Content
Don’t take risks with your media. If your sterile filtration membranes are clogging and/or binding serum proteins in your media, your cells risk: Cellular damage, Exposure to toxins and Suboptimal cell growth. Trust Stericup® filters with NEW improved Millipore Express® PLUS membrane. Our most popular Stericup® membrane, Millipore Express® PLUS polyethersulfone (PES), now delivers even faster filtration rates while reducing overall protein and reagent loss.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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