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Merck

SCCE016

Sigma-Aldrich

Human Corneal Epithelial Cells

Human Corneal Epithelial Cells provide an ideal serum-free culture model without antimicrobials or phenol red when used with the EpiGRO Ocular Epithelia Complete Media Kit.

Synonym(s):

HCEC cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.71

Key Documents

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biological source

human cornea (epithelial cells)

Quality Level

100

packaging

vial of 500,000 cells

manufacturer/tradename

Chemicon®
EpiGRO®
Millipore

morphology

(epithelial)

technique(s)

cell culture | mammalian: suitable

shipped in

liquid nitrogen

General description

Human Corneal Epithelial Cells may be used for studies of cell-matrix interactions, gene regulation and tissue development, drug development, and validation of alternative methods in toxicology. The Human Corneal Epithelial Cells are cryopreserved as secondary cells. The cells have been isolated from human corneal tissue and expanded twice in culture vessels before being harvested for cryopreservation. This product is for Research Use Only. This product is not approved for human or veterinary use, or for use in in vitro diagnostic or clinical procedures.
Product Source: Human Corneal Epithelia

Application

Please see data sheet for detailed directions on Thawing, Plating , Feeding, Passaging, etc.
Research Category
Stem Cell Research

Biochem/physiol Actions

Cornea
Epithelial Cells

Packaging

500,000 cells

Preparation Note

To maintain the cells’ integrity, Millipore recommends unpacking the products immediately upon receipt and storing at a temperature lower than -150°C or in liquid nitrogen vapor phase.

Analysis Note

Cells are negative for HIV (1,2), HBV, HCV, bacteria, mycoplasma and fungal contamination.

Further growth: Guaranteed to provide 3 or more passages when grown in the appropriate EpiGRO Medium.

Other Notes

SCCE016

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
EPIGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adrian Gericke et al.
RSC advances, 9(39), 22531-22539 (2019-07-22)
Impaired regeneration of the corneal epithelium, as found in many ocular surface diseases, is a major clinical problem in ophthalmology. We hypothesized that corneal epithelial regeneration can be promoted by the physiological, energy-delivering as well as "morphogenetically active" polymer, inorganic

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EpiGRO™, EndoGRO™ and FibroGRO™ Reagents

"The successful, reliable culture of epithelial cells is critical for many areas of research, including dermatology, respiratory research, and cancer research. Because the breakdown of control mechanisms in epithelial cells is a frequent contributor to cancer progression and metastasis, epithelial cell culture is particularly important for cancer research. EpiGRO™ media formulations are optimized to provide better viability, proliferation rates, morphology and culture stability than other commercially available options. The media are provided in unique, light-blocking, temperature-monitored packaging to ensure stability and protect the media from damage by light, contamination, and excessive heat. The media do not require or contain any antimicrobials or phenol red. These components can cause cell stress and influence experimental results by masking the true performance or health of the cell culture. Phenol red acts like an estrogen and may stimulate growth independently of experimental variables. "

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