PEZGS0416
Millicell® EZ Slide
4-well glass chamber slide, 1.7 cm2/well, 16/pk, sterile
Synonym(s):
4 well glass slide, Chamber slides
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About This Item
material
polypropylene wells
polystyrene lid
soda-lime glass slide
Quality Level
sterility
ethylene oxide treated
sterile
feature
Raised corner ridges on the lid facilitate stacking of multiple units
packaging
box of 16 (4 trays, 4 units/tray)
manufacturer/tradename
Millicell®
technique(s)
cell based assay: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
L × W × H
75 mm × 25 mm × 1 mm, slide only
77.8 mm × 33.8 mm × 13.9, excluding lid
L × W
77.8 mm × 33.8 mm × 16.2 mm, including lid
size
4 wells
well surface area
1.7 cm2
well volume
500-1700 μL
shipped in
ambient
Related Categories
General description
Preparation Note
- Culture, fix, and stain the cells according to preferred protocol.
- To access the microscope slide, hold the Millicell® EZ SLIDE down on a flat surface and break the four tabs by pushing them down with the thumb. Remove the cover. Hold the slide down and lift the wells upward to remove. Remove slide from holder.
Legal Information
Certificates of Analysis (COA)
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Articles
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Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.
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