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Merck

MAB2137

Sigma-Aldrich

Anti-Homing Receptor Antibody, clone A020

clone A020, Chemicon®, from rat

Synonym(s):

CD44s, HCAM, Pgp-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rat

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

A020, monoclonal

species reactivity

mouse, human, rat, pig, rabbit, canine, bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_000610.3
NM_001001389.1
NM_001001390.1
NM_001001391.1
NM_001001392.1

UniProt accession no.

P16070

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CD44(960)

General description

90 kDa

Immunogen

Purified human blood lymphocyte CD44

Application

Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.
ELISA 1:2000

Flow Cytometry :1:1000

Immunofluorescence 1:200

Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

Immunoprecipitation 1:200

Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Biochem/physiol Actions

Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).

Physical form

Format: Purified
Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.
Protein A purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Cells of epithelium and highly expressed by carcinomas

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Z Gilcrease et al.
Cancer, 86(11), 2320-2326 (1999-12-11)
Recent reports have shown altered expression of CD44 in renal cell carcinomas. However, to the authors' knowledge there are no data correlating CD44 expression in renal cell carcinomas with subsequent tumor progression or recurrence, nor is there information about the
Milan Makwana et al.
The Journal of comparative neurology, 518(5), 699-721 (2009-12-25)
Generation of new axonal sprouts plays an important role in neural repair. In the current study, we examined the appearance, composition and effects of gene deletions on intrabrainstem sprouts following peripheral facial nerve axotomy. Axotomy was followed by the appearance
Mature human thymocytes migrate on laminin-5 with activation of metalloproteinase-14 and cleavage of CD44.
Vivinus-Nebot, Mylene, et al.
Journal of immunology (Baltimore, Md. : 1950), 172, 1397-1406 (2004)
Sameerah Shaheen et al.
Stem cell reviews and reports, 12(4), 492-499 (2016-05-22)
Colorectal cancers (CRCs) form a disorganized hierarchy of heterogeneous cell populations on which current chemotherapy regimens fail to exert their distinctive cytotoxicity. A small sub-population of poorly differentiated cancer stem-like cells (CSCs), also known as cancer initiating cells, may exhibit
Carmela Ricciardelli et al.
BMC cancer, 13, 476-476 (2013-10-16)
Hyaluronan (HA) an important component of the extracellular matrix, has been linked to tumor progression and drug resistance in several malignancies. However, limited data is available for ovarian cancer. This study investigated the role of hyaluronan (HA) and a potential
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Anti-Homing Receptor, clone A020 - Data Sheet

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