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Merck

IPFL85R

Millipore

Immobilon®-FL PVDF Membrane

1 roll, 8.5cm x 10m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Synonym(s):

Immobilon NOW, Immobilon narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23
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Product Name

Immobilon®-FL PVDF, 0.45 m, 8.5 cm x 10 m roll, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Fluorescent, Chemifluorescence, Chromogenic, or Chemiluminescence. Comes in a 8.5 cm x 10 m roll.

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with All fluorescence dyes
for use with Amido black
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

General description

Filter Code: IPFL

Application

Immobilon®-FL PVDF, 0.45 m, 8.5 cm x 10 m roll has been used in westernblotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

폴리아크릴아미드 겔 화학의 개요와 mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 폴리아크릴아미드 겔 핸드캐스팅에 대한 상세 지침

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Related Content

이해합니다. 서양인들은 얼룩으로 인해 고통스러울 수 있으며, 저희도 확실히 그 고통을 겪었습니다. 그러니 여러분의 못생긴 오점을 공유해 주시고 깜짝 선물을 받아보세요! 깜짝 선물이 무엇인지 알려드릴 수는 없지만, 실험실에서 창의력을 자극할 수 있다는 점만 말씀드릴 수 있습니다.

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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