Skip to Content
Merck

Key Documents

View All Documentation

5010-OP

Guanidine Hydrochloride

OmniPur® Grade, >= 99.5%, Denaturant used for purification of nucleic acids and polypeptides from cell lysates

Synonym(s):

Guanidine hydrochloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
50-01-1
Molecular Weight:
95.53
NACRES:
NA.28
UNSPSC Code:
12352107
Beilstein/REAXYS Number:
3591990
MDL number:
MFCD00013026
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

100

form

crystalline powder (granular)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

mp

180-185 °C (lit.)

density

1.3 g/cm3 (lit.)

foreign activity

DNAse, none detected, Phosphatase, none detected, Protease, none detected, RNAse, none detected

shipped in

ambient

storage temp.

10-30°C

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

General description



Denaturant used for purification of nucleic acids and polypeptides from cell lysates. Inhibits RNase and protease activity. Molecular Biology grade.br
A260 (0.1 M, water): ≤0.05 AU
A280 (0.1 M, water): ≤0.01 AU
Purity by Assay: ≥99.5%
DNase: None detected
Heavy metals (as Pb): ≤0.001%
Phosphatase: None detected
Protease: None detected
RNase: None detected





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under Guanidine Hydrochloride

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKUGTIN
5020-10KG04055977242256
5010-1KG07790788050849
5030-5KG07790788050856