Skip to Content
Merck

492016

Millipore

NP-40 Alternative

Synonym(s):

NP-40 Alternative - CAS 9016-45-9 - Calbiochem, Nonylphenyl Polyethylene Glycol

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

CAS Number:
9016-45-9
MDL number:
MFCD00132851
UNSPSC Code:
12161902
NACRES:
NA.25

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

description

RTECS - MD0905000

Quality Level

200

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

clear colorless

CMC

50 - 300 μM

solubility

water: 25 mg/mL
aqueous buffer: soluble

density

1.06 g/mL

application(s)

(Isolating membrane proteins)

shipped in

ambient

storage temp.

15-25°C

SMILES string

O(CCOc1ccc(cc1)CCCCCCCCC)CCO

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

Related Categories

General description

A non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis. Similar in usage to NP-40 and TRITON™ X-100 Detergent(Cat. No. 648462).

Application

NP-40 Alternative has been used:
  • as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
  • as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
  • for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Irritant (B)

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Susan H Eshleman et al.
Cell reports, 27(5), 1422-1433 (2019-05-03)
This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that
San-Ha Lee et al.
Chemical & pharmaceutical bulletin, 67(9), 966-976 (2019-07-02)
Honokiol, a biphenolic neolignan isolated from Magnolia officinalis, was reported to have a promising anti-inflammatory activity for the treatment of various diseases. There are many efforts on the synthesis and structure-activity relationship of honokiol derivatives. However, regioselective O-alkylation of honokiol
Peiwei Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(5), E934-E943 (2018-01-18)
Bardet-Biedl syndrome (BBS) is a ciliopathy resulting from defects in the BBSome, a conserved protein complex. BBSome mutations affect ciliary membrane composition, impairing cilia-based signaling. The mechanism by which the BBSome regulates ciliary membrane content remains unknown. Chlamydomonas bbs mutants
Arnab Mukhopadhyay et al.
Nature protocols, 3(4), 698-709 (2008-04-05)
In order to determine how signaling pathways differentially regulate gene expression, it is necessary to identify the interactions between transcription factors (TFs) and their cognate cis-regulatory DNA elements. Here, we have outlined a chromatin immunoprecipitation (ChIP) protocol for use in
Gustav Roder et al.
European journal of immunology, 39(10), 2682-2694 (2009-09-04)
Tapasin (Tpn) is an ER chaperone that is uniquely dedicated to MHC-I biosynthesis. It binds MHC-I molecules, integrates them into peptide-loading complexes, and exerts quality control of the bound peptides; only when an "optimal peptide" is bound will the MHC-I
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service