03-0191-00
SMC® Human IFN-β1 High Sensitivity Immunoassay Kit
Synonym(s):
Interferon β-1 immunoassay kit, Interferon beta-1 Single Molecule Counting Kit, Interferon beta-1 immunoassay kit, Interferon beta-1 ultra-sensitive immunoassay
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About This Item
description
0.07-0.30 pg/mL, refer to CoA for lot-specific LLOQ
manufacturer/tradename
SMC®
assay range
sensitivity: 0.15 pg/mL
(lower limit of quantification)
inter-assay cv: 13%
intra-assay cv: 6%
technique(s)
ELISA: suitable
single molecule counting: suitable
relevant disease(s)
infectious diseases; autoimmune diseases; neurodegenerative disorders
storage temp.
2-8°C
General description
The SMC® Human IFN-β1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Application
- Inflammation & Immunology
- Immune Response
- Infectious Diseases
- Signaling
- Neuroscience
Research Sub-Category:
- Inflammatory Disease
- Immune Response
- Autoimmune disease
- Neurodegenerative Diseases
- Neuroregenerative Medicine
- Virology
- Infectious Diseases - Viral
- Immunological Signaling
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IFN-β1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-β1 in human serum and plasma.
Biochem/physiol Actions
Cross-reactivity to the following analytes were tested with the following results:
- human IFN-α2 – 0.2%
- human IFN-γ – 0.3%
- human IL-29 (IFN-λ1) – 0.2%
- human IL-28A (IFN-λ2) – 0.2%
- human IL-28B (IFN-λ3) – 0.3%
- human IFN-ω – 0.2%
Accuracy:
- Minimum of 5 serum samples spiked with average recovery of 88% with a range of 78-100%
- Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 75-113%
Features and Benefits
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Packaging
Other Notes
- Assay Buffer
- IFN-β1 Coated Beads
- Standard Diluent
- IFN-β1 Detection Antibody
- IFN-β1 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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