L2897
LB Broth with agar (Lennox)
Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
Synonym(s):
Lennox broth, Luria Bertani Agar
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.
Application
LB Broth with agar (Lennox) has been used for:
- the transformation of competent E. coli cells
- to cultivate Ecc-15 bacteria
- embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
- transmission electron microscopy (TEM) and immunohistochemistry (IHC)
Features and Benefits
Lennox LB powder with agar provides:
- A budget-friendly alternative to pre-poured plates
- Easy scale-up using larger package sizes
- Standard formulation
Preparation Note
1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
General protocols for growth of competent cells in microbial medium.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| L2897-1KG | 04061835573400 |
| L2897-250G | 04061835573417 |