Skip to Content
Merck

G8529

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

CAS Number:
9001-40-5
EC Number:
1.1.1.49 (BRENDA, IUBMB)
EC Number:
232-602-6
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in E. coli

Quality Level

300

form

lyophilized powder

specific activity

≥550 units/mg protein (biuret)

composition

Protein, 10-40% biuret

application(s)

agriculture

foreign activity

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:

  • in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
  • to study the starch time course using Nicotiana tabacum leaves
  • to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
  • to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
079M4188V
059M4023V
50190302
069M4002V
039M4071V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Alyssa L Preiser et al.
Frontiers in plant science, 11, 580726-580726 (2020-12-29)
Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of
Ching Man Wai et al.
PLoS genetics, 15(6), e1008209-e1008209 (2019-06-15)
Plants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks
Thomas Beneyton et al.
Nature communications, 9(1), 2391-2391 (2018-06-21)
Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and
Myriam Ferrara et al.
Extremophiles : life under extreme conditions, 17(1), 53-62 (2012-11-03)
Psychrophilic organisms have evolved a number of modifications of cellular structures to survive in the cold environment; among them it is worth noting an increased efficiency of enzymes at lower temperatures. Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) was purified and characterised
Shinichi Harada et al.
The Journal of pharmacology and experimental therapeutics, 344(1), 276-285 (2012-11-03)
Orexin-A (a glucose-sensing neuropeptide in the hypothalamus) and brain-derived neurotrophic factor (BDNF; a member of the neurotrophin family) play roles in many physiologic functions, including regulation of glucose metabolism. We previously showed that the development of postischemic glucose intolerance is
View All Related Papers

Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Related Content

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service