A9469
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
alkaline phosphatase conjugate
Clone:
M2, monoclonal
Application:
ELISA (i)
Citations:
34
biological source
mouse
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
indirect ELISA: 1:20,000
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... ALPL(249)
General description
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:
- in direct tissue blot immunoassay of sweet orange petioles samples
- in screening internalization of delta opioid receptor
- for screening cell-free protein expression using ELISA
Physical form
Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Matthew T Doherty et al.
The Journal of biological chemistry, 287(47), 39369-39379 (2012-10-06)
Myb repeats ∼52 amino acid residues in length were first characterized in the oncogenic Myb transcription factor, which contains three tandem Myb repeats in its DNA-binding domain. Proteins of this family normally contain either one, two, or three tandem Myb
The delta opioid receptor positive allosteric modulator BMS 986187 is a G protein biased allosteric agonist
Stanczyk MA, et al.
British Journal of Pharmacology (2019)
Fang Ding et al.
Scientific reports, 6, 29272-29272 (2016-07-07)
We describe experiments with antibodies against 'Candidatus Liberibacter asiaticus used to detect the pathogen in infected plants. We used scFv selected to bind epitopes exposed on the surface of the bacterium in tissue prints, with secondary monoclonal antibodies directed at
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9469-5X1MG | 04061829673192 |
| A9469-.2MG | 04061835504541 |
| A9469-1MG | 04061826671115 |