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Merck

41698

Sigma-Aldrich

Atto 488 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Atto 488

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

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product line

BioReagent

Quality Level

100

Assay

≥90% (HPLC)
≥90% (degree of coupling)

form

powder

mol wt

686.66 g/mol

manufacturer/tradename

ATTO-TEC GmbH

color

orange
 red

λmax

501-507 nm in methanol: water (1:1) (with 0.1% perchloric acid)

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 501-507 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CN(CCCC(=O)ON1C(=O)CCC1=O)C(=O)C2=CC=CC=C2C3=C4C=CC(=[NH2+])C(=C4OC5=C3C=CC(=C5S(=O)(=O)[O-])N)S(=O)(=O)O

InChI

InChI=1S/C29H26N4O12S2/c1-32(14-4-7-23(36)45-33-21(34)12-13-22(33)35)29(37)16-6-3-2-5-15(16)24-17-8-10-19(30)27(46(38,39)40)25(17)44-26-18(24)9-11-20(31)28(26)47(41,42)43/h2-3,5-6,8-11,30H,4,7,12-14,31H2,1H3,(H,38,39,40)(H,41,42,43)

InChI key

QYLVUXZLEBHDMK-UHFFFAOYSA-N

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General description

Atto 488 NHS ester is a hydrophilic fluorescent label with high water solubility. The fluorescence activity is excited efficiently at the 480-515nm range. A suitable excitation source for Atto 488 is the 488 nm line of the Argon-Ion laser.

Application

Atto 488 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Features and Benefits

Characteristic features of the Atto 488 NHS ester are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Excellent water solubility.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5527
BCCK2510
BCCK4443
BCCK0539
BCCJ6663

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Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Muller, P., et al. et al.
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
Qian Peter Su et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15036-15046 (2020-06-17)
Mammalian DNA replication is initiated at numerous replication origins, which are clustered into thousands of replication domains (RDs) across the genome. However, it remains unclear whether the replication origins within each RD are activated stochastically or preferentially near certain chromatin
Switching modulation for protein labeling with activatable fluorescent probes.
Kalyan K Sadhu et al.
Chembiochem : a European journal of chemical biology, 12(9), 1299-1308 (2011-06-02)
Christiane Iserman et al.
Molecular cell, 80(6), 1078-1091 (2020-12-09)
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic elements under physiological buffer conditions and condensation is enhanced at human body temperatures (33°C and 37°C) and reduced
Gražvydas Lukinavičius et al.
Current opinion in chemical biology, 15(6), 768-774 (2011-11-15)
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins
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