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Merck

189490

Sigma-Aldrich

3-MA

≥95% (HPLC), powder, autophagy inhibitor, Calbiochem®

Synonym(s):

Autophagy Inhibitor, 3-MA, 3-Methyladenine

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About This Item

Empirical Formula (Hill Notation):
C6H7N5
CAS Number:
5142-23-4
Molecular Weight:
149.15
MDL number:
MFCD00010531
UNSPSC Code:
12352200
NACRES:
NA.77

Key Documents

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Product Name

Autophagy Inhibitor, 3-MA, Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker. Acts as an inhibitor of III PI3-Kinase.

Quality Level

100

Assay

≥95% (HPLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white to faint yellow

solubility

DMSO: 10 mg/mL
water: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

SMILES string

N1(C2=NC=NC2=C(N=C1)N)C

InChI

1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

InChI key

FSASIHFSFGAIJM-UHFFFAOYSA-N

Related Categories

General description

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204 ) and Wortmannin (Cat. No. 681675 )

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.

Other Notes

Canu, N., et al. 2005. J. Neurochem.92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.
Beugnet, A., et al. 2003. Biochem. J.372, 555.
Eskelinen, E.L., et al. 2002. Traffic3, 878.
Petiot, A., et al. 2000. J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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