Key Documents

View All Documentation

G0762

β-Glucuronidase from Helix pomatia

Name: β-Glucuronidase from <I>Helix pomatia</I>
Brand: SIGMA

Type H-3AF, aqueous solution, ≥60,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

Select a Size

Change View

About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.31 (BRENDA, IUBMB)

MDL number:

MFCD00130629

Specific activity:

≥60,000 units/mL

Biological source:

Helix pomatia

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

Helix pomatia

Quality Level

300

type

Type H-3AF

form

aqueous solution

specific activity

≥60,000 units/mL

impurities

≤1,000 unit/mL Sulfatase

shipped in

wet ice

storage temp.

2-8°C

Description

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

A further processing of Type H-3 to remove agglutinin.

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Still not finding the right product?

Explore all of our products under β-Glucuronidase from Helix pomatia

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

7783-20-2

CAS No.

rmu

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Correlation of oestriol levels in saliva, plasma and urine of pregnant women.

W Lechner et al.

Acta endocrinologica, 109(2), 266-268 (1985-06-01)

Unconjugated and total oestriol (E3) have been determined in saliva (S) and plasma (P) by radioimmunoassay using a highly specific antiserum against E3. Total oestrogens (E = E1 + E2 + E3) in urine were analysed by the Kober-Ittrich method.

Antitumor efficacy of the heparanase inhibitor SST0001 alone and in combination with antiangiogenic agents in the treatment of human pediatric sarcoma models.

G Cassinelli et al.

Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)

The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded

Lysosomal β-glucuronidase regulates Lyme and rheumatoid arthritis severity.

Kenneth K C Bramwell et al.

The Journal of clinical investigation, 124(1), 311-320 (2013-12-18)

Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most prevalent arthropod-borne illness in the United States and remains a clinical and social challenge. The spectrum of disease severity among infected patients suggests that host genetics contribute to pathogenic

View All Related Papers

Global Trade Item Number

SKU	GTIN
G0762-100KU	04061833624265