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Merck

F9378

Ficoll® 400

Type 400-DL, lyophilized powder

Synonym(s):

Polysucrose 400

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About This Item

CAS Number:
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
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biological source

sugar beets (Beta vulgaris)

Quality Level

type

Type 400-DL

form

lyophilized powder

color

white

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

storage temp.

15-25°C

Application

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. Ficoll 400 may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.
Used for cell separation and organelle isolation.

Other Notes

A nonionic synthetic polymer of sucrose.
Dialyzed
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Legal Information

Ficoll is a registered trademark of Cytiva


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.


Mirna González-González et al.
Biotechnology progress, 30(3), 700-707 (2014-01-23)
Aqueous two-phase systems (ATPS) represent a promising strategy for the recovery of CD133(+) stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon)
Bo Chen et al.
Tissue engineering. Part C, Methods, 19(8), 586-595 (2013-02-21)
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is
Lucie Kosinova et al.
Islets, 11(6), 129-140 (2019-09-10)
Instant Blood-Mediated Inflammatory Reaction (IBMIR) is a major cause of graft loss during pancreatic islet transplantation, leading to a low efficiency of this treatment method and significantly limiting its broader clinical use. Within the procedure, transplanted islets obstruct intrahepatic portal



Global Trade Item Number

SKUGTIN
F9378-100G04061835366613
F9378-10G04061835366620
F9378-500G04061833621585
F9378-25G04061833621547
F9378-5G04061835366637