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Merck

F8192

Fetal Bovine Serum

Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

HI FBS, HI sera, HI serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
Australia origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions



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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.


Leon M Larcher et al.
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Jiamin Huang et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 69(3), 321-330 (2020-02-07)
To reveal the systematic response of neutrophils to sepsis and to study the hub lncRNAs in sepsis. Neutrophils taken from the femur and tibia of male C57 BL/6 mice were used in this study. And neutrophils were treated for 0 h
Marti Cabanes-Creus et al.
Molecular therapy. Methods & clinical development, 12, 71-84 (2018-12-12)
Adeno-associated virus (AAV) vectors have become one of the most widely used gene transfer tools in human gene therapy. Considerable effort is currently being focused on AAV capsid engineering strategies with the aim of developing novel variants with enhanced tropism



Global Trade Item Number

SKUGTIN
F8192-500ML04061833620403
F8192-50ML04061838113719
F8192-100ML04061833620380
F8192-1L04061838113702