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A1602

Amyloglucosidase from Aspergillus niger

Name: Amyloglucosidase from <I>Aspergillus niger</I>
Brand: SIGMA

ammonium sulfate suspension, ≥40 units/mg protein

Synonym(s):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

CAS Number:

9032-08-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00081350

Specific activity:

≥40 units/mg protein

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Properties

form

ammonium sulfate suspension

Quality Level

200

specific activity

≥40 units/mg protein

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH approx. 6.0

Other Notes

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

7783-20-2

CAS No.

rmu

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Certificates of Analysis (COA)

Lot/Batch Number

037K7405

102K7500

065K7455

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Protocols

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Global Trade Item Number

SKU	GTIN
A1602-100MG	04061835540518
A1602-25MG	04061835551095