XNAPG
SYBR® Green Extract-N-Amp™ Plant PCR Kit
sufficient for 100 preparations
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About This Item
Quality Level
usage
sufficient for 100 preparations, sufficient for 100 reactions
feature
dNTPs included, hotstart
technique(s)
RT-qPCR: suitable, qPCR: suitable
input
crude DNA
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
General description
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
Features and Benefits
- Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- No loading buffers or tracking dyes required for gel analysis
- SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Compatible with high-throughput requirements for genetic analysis of plants
- No long enzymatic digestions
- Extract stable at 4°C for at least 6 months
Other Notes
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Articles
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Die Verfügbarkeit von einfachen Verfahren zum Aufreinigen von DNA und RNA hat die Analyse und Charakterisierung des Genoms und der Genexpression in hohem Maß erleichtert. Es besteht eine Anforderung, DNA und RNA schnell und praktisch aus verschiedenen zellulären Quellen, einschließlich Gewebe aus Tier-, Pflanzen- und Bakterienkulturen, zu isolieren.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| XNAPG-1KT | 04061837549915 |